MoP-07
APPLICATIONS OF FOURIER TRANSFORM MASS SPECTROMETRY (FTMS) TO THE ANALYSIS OF COMBINATORIAL LIBRARIES
G. H. Kruppa, F. H. Laukien, J. Wronka, J. P. Speir and T. Zhang
Bruker Analytical Systems Inc., Billerica, MA, USA
Combinatorial chemistry can produce libraries of compounds with hundreds or even thousands of compounds. There has been great interest in the analytical community for methods that can rapidly screen these libraries to determine the number and identity of compounds that are actually present. Ideally the fastest methods would not involve a pre-separation step, but automated methods involving chromatographic pre-separation have also been of interest. Recent results from the application of FTMS to the analysis of these types of libraries will be presented. Examples will be shown where the high resolving power and mass accuracy of FTMS can be used to analyze complex combinatorial libraries, both with and without chromatographic separation. In addition the application of MS/MS and isotopic distributions from FTMS to further confirm the identity of library components will be discussed.
Discrimination effects due to different ionization efficiencies of the various components in a library, and also due to matrix effects, have made it difficult to be sure that all components in a library are observed without chromatographic separation. To overcome this difficulty, the use of different and complementary ionization methods has been investigated. The use of a combination of ionization methods to increase the number of observed components will be shown, including positive and negative ion ESI, and MALDI.