MoP-04
AUTOMATED PEPTIDE MASS SCREENING AND PROTEIN IDENTIFICATION USING A HYBRID QUADRUPOLE-ORTHOGONAL TIME OF FLIGHT (QTOF) AND MALDI-TOF MASS SPECTROMETER COMBINED WITH DATABASE SEARCHING STRATEGIES
J. I. Langridge, R. S. Bordoli, T McKenna, E. Kapp, D. Stranz and G. Critchley
Micromass UK Ltd., Tudor Road, Altrincham, WA14 5RZ, UK
Integrated and automated techniques for protein analysis and data interpretation are essential for rapid protein characterisation and the proteome project. Searching protein sequence databases using mass spectrometry information forms an integral part of this project and allows rapid screening and identification of known proteins or contaminants. Any strategy designed to uniquely identify proteins must provide unambiguous results without false positives. To achieve the high throughput, as well as sensitive and certain identification, almost complete sequence coverage of a particular protein should be obtained.
Both nanoflow coupled to an electrospray ionisation source on a hybrid Quadropole-orthogonal acceleration Time of Flight and MALDI-TOF mass spectrometer using Time-Lag Focussing can be integrated with a powerful database search engine (ProteinProbe) to provide the sensitivity and specificity required for total protein characterisation.
The results of different search strategies are presented using data obtained from the analysis of unseparated digest mixtures by Nanoflow ESI and MALDI-TOF.